ARHGDIA ELISA Kit

Catalog No. ABIN5658486

Product Details ARHGDIA ELISA Kit

Target: ARHGDIA (rho GDP Dissociation Inhibitor (GDI) alpha (ARHGDIA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Rho GDP Dissociation Inhibitor Alpha (ARHGDIa). No significant cross-reactivity or interference between Rho GDP Dissociation Inhibitor Alpha (ARHGDIa) and analogues was observed.

Sensitivity: 0.066 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rho GDP Dissociation Inhibitor Alpha (ARHGDIa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rho GDP Dissociation Inhibitor Alpha (ARHGDIa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rho GDP Dissociation Inhibitor Alpha (ARHGDIa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rho GDP Dissociation Inhibitor Alpha (ARHGDIa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rho GDP Dissociation Inhibitor Alpha (ARHGDIa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rho GDP Dissociation Inhibitor Alpha (ARHGDIa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ARHGDIA

Target: ARHGDIA (rho GDP Dissociation Inhibitor (GDI) alpha (ARHGDIA))

Alternative Name: Rho GDP Dissociation Inhibitor Alpha (ARHGDIA Products)

Synonyms: GDIA1 ELISA Kit, NPHS8 ELISA Kit, RHOGDI ELISA Kit, RHOGDI-1 ELISA Kit, Bles01 ELISA Kit, zgc:55554 ELISA Kit, zgc:77681 ELISA Kit, wu:fa96g11 ELISA Kit, ARHGDIA ELISA Kit, NV15502 ELISA Kit, 5330430M07Rik ELISA Kit, Gdi-1 ELISA Kit, Rho-GDI ELISA Kit, RhoDGI ELISA Kit, RhoGDI-1 ELISA Kit, arhgdia ELISA Kit, MGC84535 ELISA Kit, gdia1 ELISA Kit, rhogdi ELISA Kit, rhogdi-1 ELISA Kit, rhogdi1 ELISA Kit, Rho GDP dissociation inhibitor alpha ELISA Kit, Rho GDP dissociation inhibitor (GDI) alpha ELISA Kit, rho GDP-dissociation inhibitor 2 ELISA Kit, Rho GDP dissociation inhibitor (GDI) alpha L homeolog ELISA Kit, ARHGDIA ELISA Kit, Arhgdia ELISA Kit, arhgdia ELISA Kit, LOC100120432 ELISA Kit, arhgdia.L ELISA Kit

Background:

Gene Name: Rho GDP Dissociation Inhibitor Alpha

Gene Aliases: GDIA1, RHOGDI, RHOGDI-1

Pathways: Neurotrophin Signaling Pathway