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RYR2 ELISA Kit

RYR2 Reactivity: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Tissue Homogenate
Catalog No. ABIN5658537
  • Target See all RYR2 ELISA Kits
    RYR2 (Ryanodine Receptor 2 (Cardiac) (RYR2))
    Reactivity
    • 2
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.312 ng/mL - 20 ng/mL
    Minimum Detection Limit
    0.312 ng/mL
    Application
    ELISA
    Sample Type
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Ryanodine Receptor 2, Cardiac (RYR2). No significant cross-reactivity or interference between Ryanodine Receptor 2, Cardiac (RYR2) and analogues was observed.
    Sensitivity
    0.107 ng/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ryanodine Receptor 2, Cardiac (RYR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ryanodine Receptor 2, Cardiac (RYR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ryanodine Receptor 2, Cardiac (RYR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ryanodine Receptor 2, Cardiac (RYR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ryanodine Receptor 2, Cardiac (RYR2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ryanodine Receptor 2, Cardiac (RYR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all RYR2 ELISA Kits
    RYR2 (Ryanodine Receptor 2 (Cardiac) (RYR2))
    Alternative Name
    Ryanodine Receptor 2, Cardiac (RYR2 Products)
    Synonyms
    RYR2 ELISA Kit, ARVC2 ELISA Kit, ARVD2 ELISA Kit, RYR-2 ELISA Kit, RyR ELISA Kit, VTSIP ELISA Kit, 9330127I20Rik ELISA Kit, ryanodine receptor 2 ELISA Kit, ryanodine receptor 2, cardiac ELISA Kit, RYR2 ELISA Kit, Ryr2 ELISA Kit
    Background

    Gene Name: Ryanodine Receptor 2, Cardiac

    Gene Aliases: RYR-2, ARVD2, ARVC2, VTSIP, Arrhythmogenic Right Ventricular Dysplasia 2, Cardiac muscle ryanodine receptor-calcium release channel

    Pathways
    Myometrial Relaxation and Contraction
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