SPR ELISA Kit

Catalog No. ABIN5658711

Product Details SPR ELISA Kit

Target: SPR (Sepiapterin Reductase (SPR))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Sepiapterin Reductase (SPR). No significant cross-reactivity or interference between Sepiapterin Reductase (SPR) and analogues was observed.

Sensitivity: 0.061 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sepiapterin Reductase (SPR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sepiapterin Reductase (SPR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sepiapterin Reductase (SPR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sepiapterin Reductase (SPR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sepiapterin Reductase (SPR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sepiapterin Reductase (SPR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SPR

Target: SPR (Sepiapterin Reductase (SPR))

Alternative Name: Sepiapterin Reductase (SPR Products)

Synonyms: SDR38C1 ELISA Kit, AA409688 ELISA Kit, ENSMUSG00000071345 ELISA Kit, Gm10328 ELISA Kit, CG12117 ELISA Kit, Dmel\\CG12117 ELISA Kit, SR ELISA Kit, sptr ELISA Kit, xspr ELISA Kit, DDBDRAFT_0188686 ELISA Kit, DDBDRAFT_0191275 ELISA Kit, DDB_0188686 ELISA Kit, DDB_0191275 ELISA Kit, zgc:194446 ELISA Kit, sepiapterin reductase ELISA Kit, Sepiapterin reductase ELISA Kit, sepiapterin reductase L homeolog ELISA Kit, sepiapterin reductase b ELISA Kit, SPR ELISA Kit, Spr ELISA Kit, Sptr ELISA Kit, spr.L ELISA Kit, sprE ELISA Kit, CpipJ_CPIJ000511 ELISA Kit, sr ELISA Kit, spr ELISA Kit, sprb ELISA Kit

Background:

Gene Name: Sepiapterin Reductase

Gene Aliases: SDR38C1, 7,8-Dihydrobiopterin:NADP+ Oxidoreductase, Short Chain Dehydrogenase/Reductase Family 38C,Member 1

UniProt: Q64105

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration