SOX9 ELISA Kit

Catalog No. ABIN5658768

Product Details SOX9 ELISA Kit

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Sex Determining Region Y Box Protein 9 (SOX9). No significant cross-reactivity or interference between Sex Determining Region Y Box Protein 9 (SOX9) and analogues was observed.

Sensitivity: 0.129 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sex Determining Region Y Box Protein 9 (SOX9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sex Determining Region Y Box Protein 9 (SOX9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sex Determining Region Y Box Protein 9 (SOX9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sex Determining Region Y Box Protein 9 (SOX9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sex Determining Region Y Box Protein 9 (SOX9) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sex Determining Region Y Box Protein 9 (SOX9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SOX9

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Alternative Name: Sex Determining Region Y Box Protein 9 (SOX9 Products)

Synonyms: CMD1 ELISA Kit, CMPD1 ELISA Kit, SRA1 ELISA Kit, LOC100227849 ELISA Kit, 2010306G03Rik ELISA Kit, AV220920 ELISA Kit, mKIAA4243 ELISA Kit, SOX-9 ELISA Kit, cmd1 ELISA Kit, cmpd1 ELISA Kit, sox-9 ELISA Kit, sox9 ELISA Kit, sox9-a ELISA Kit, sox9-b ELISA Kit, sox9b ELISA Kit, sra1 ELISA Kit, SRY-box 9 ELISA Kit, transcription factor SOX-9 ELISA Kit, SRY (sex determining region Y)-box 9 ELISA Kit, SRY box 9 ELISA Kit, SRY-box 9 L homeolog ELISA Kit, SOX9 ELISA Kit, LOC100227849 ELISA Kit, Sox9 ELISA Kit, sox9.L ELISA Kit

Background:

Gene Name: Sex Determining Region Y Box Protein 9

Gene Aliases: CMPD1, CMD1, SRA1, Campomelic Dysplasia,Autosomal Sex-Reversal, Transcription factor SOX-9

Pathways: EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development