Sortilin 1 ELISA Kit

Catalog No. ABIN5658895

Product Details Sortilin 1 ELISA Kit

Target: Sortilin 1 (SORT1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Sortilin 1 (SORT1). No significant cross-reactivity or interference between Sortilin 1 (SORT1) and analogues was observed.

Sensitivity: 0.064 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sortilin 1 (SORT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sortilin 1 (SORT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sortilin 1 (SORT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sortilin 1 (SORT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sortilin 1 (SORT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sortilin 1 (SORT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Sortilin 1

Target: Sortilin 1 (SORT1)

Alternative Name: Sortilin 1 (SORT1 Products)

Synonyms: SORT1 ELISA Kit, 19.m02079 ELISA Kit, gp95 ELISA Kit, ldlcq6 ELISA Kit, sortilin ELISA Kit, Gp95 ELISA Kit, LDLCQ6 ELISA Kit, NT3 ELISA Kit, 2900053A11Rik ELISA Kit, AI852375 ELISA Kit, Ntr3 ELISA Kit, Ntsr3 ELISA Kit, Nt3 ELISA Kit, Nts3 ELISA Kit, sort1 ELISA Kit, zgc:85953 ELISA Kit, sortilin 1 ELISA Kit, sortilin ELISA Kit, sortilin 1 S homeolog ELISA Kit, sortilin 1a ELISA Kit, SORT1 ELISA Kit, TP01_0016 ELISA Kit, BBOV_I002730 ELISA Kit, sort1.S ELISA Kit, Sort1 ELISA Kit, sort1a ELISA Kit

Background:

Gene Name: Sortilin 1

Gene Aliases: NT3, Gp95, NTR3, 100 kDa NT receptor, Glycoprotein 95, Neurotensin receptor 3

Gene ID: 6272

UniProt: Q99523

Pathways: Neurotrophin Signaling Pathway