SCD ELISA Kit

Catalog No. ABIN5658943

Product Details SCD ELISA Kit

Target: SCD (Stearoyl-CoA Desaturase (Delta-9-Desaturase) (SCD))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Stearoyl Coenzyme A Desaturase (SCD). No significant cross-reactivity or interference between Stearoyl Coenzyme A Desaturase (SCD) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stearoyl Coenzyme A Desaturase (SCD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stearoyl Coenzyme A Desaturase (SCD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stearoyl Coenzyme A Desaturase (SCD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stearoyl Coenzyme A Desaturase (SCD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stearoyl Coenzyme A Desaturase (SCD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stearoyl Coenzyme A Desaturase (SCD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SCD

Target: SCD (Stearoyl-CoA Desaturase (Delta-9-Desaturase) (SCD))

Alternative Name: Stearoyl Coenzyme A Desaturase (SCD Products)

Synonyms: FADS5 ELISA Kit, MSTP008 ELISA Kit, SCD1 ELISA Kit, SCDOS ELISA Kit, AA589638 ELISA Kit, AI265570 ELISA Kit, Scd ELISA Kit, Scd-1 ELISA Kit, ab ELISA Kit, SCD ELISA Kit, fads5 ELISA Kit, scd1 ELISA Kit, scdos ELISA Kit, F27J15.17 ELISA Kit, F27J15_17 ELISA Kit, STOMATAL CYTOKINESIS-DEFECTIVE 1 ELISA Kit, cds2 ELISA Kit, desaturase ELISA Kit, stearoyl-CoA desaturase ELISA Kit, acyl-CoA desaturase ELISA Kit, Acyl-CoA desaturase ELISA Kit, stearoyl-Coenzyme A desaturase 1 ELISA Kit, stearoyl-CoA desaturase (delta-9-desaturase) ELISA Kit, stearoyl-CoA desaturase (delta-9-desaturase) S homeolog ELISA Kit, stomatal cytokinesis defective / SCD1 protein (SCD1) ELISA Kit, acyl-CoA desaturase-like ELISA Kit, SCD ELISA Kit, CIMG_08158 ELISA Kit, VIBHAR_RS23280 ELISA Kit, acod ELISA Kit, Scd1 ELISA Kit, Scd ELISA Kit, scd ELISA Kit, LOC100346561 ELISA Kit, scd.S ELISA Kit, SCD1 ELISA Kit, LOC100719661 ELISA Kit, LOC101835884 ELISA Kit, LOC109101093 ELISA Kit

Background:

Gene Name: Stearoyl Coenzyme A Desaturase

Gene Aliases: FADS5, SCDOS, Fatty Acid desaturase 5, Delta-9-Desaturase, Stearoyl-CoA Desaturase Opposite Strand

Pathways: Brown Fat Cell Differentiation