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SUZ12 ELISA Kit

SUZ12 Reactivity: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Tissue Homogenate
Catalog No. ABIN5659017
  • Target See all SUZ12 ELISA Kits
    SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.312 ng/mL - 20 ng/mL
    Minimum Detection Limit
    0.312 ng/mL
    Application
    ELISA
    Sample Type
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Suppressor Of Zeste 12 Homolog (SUZ12). No significant cross-reactivity or interference between Suppressor Of Zeste 12 Homolog (SUZ12) and analogues was observed.
    Sensitivity
    0.113 ng/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Suppressor Of Zeste 12 Homolog (SUZ12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Suppressor Of Zeste 12 Homolog (SUZ12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Suppressor Of Zeste 12 Homolog (SUZ12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Suppressor Of Zeste 12 Homolog (SUZ12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Suppressor Of Zeste 12 Homolog (SUZ12) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Suppressor Of Zeste 12 Homolog (SUZ12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all SUZ12 ELISA Kits
    SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))
    Alternative Name
    Suppressor Of Zeste 12 Homolog (SUZ12 Products)
    Synonyms
    fc31h10 ELISA Kit, im:7153578 ELISA Kit, si:dkey-10p5.2 ELISA Kit, suz12 ELISA Kit, wu:fc31h10 ELISA Kit, 2610028O16Rik ELISA Kit, AI195385 ELISA Kit, AU016842 ELISA Kit, AW536442 ELISA Kit, D11Ertd530e ELISA Kit, mKIAA0160 ELISA Kit, Xsuz12 ELISA Kit, chet9 ELISA Kit, jjaz1 ELISA Kit, CHET9 ELISA Kit, ChET 9 protein ELISA Kit, JJAZ1 ELISA Kit, suz12l ELISA Kit, zgc:100863 ELISA Kit, SUZ12 polycomb repressive complex 2b subunit ELISA Kit, SUZ12 polycomb repressive complex 2 subunit ELISA Kit, suppressor of zeste 12 homolog (Drosophila) ELISA Kit, SUZ12 polycomb repressive complex 2 subunit S homeolog ELISA Kit, SUZ12 polycomb repressive complex 2a subunit ELISA Kit, suz12b ELISA Kit, Suz12 ELISA Kit, SUZ12 ELISA Kit, suz12.S ELISA Kit, suz12a ELISA Kit
    Background

    Gene Name: Suppressor Of Zeste 12 Homolog

    Gene Aliases: CHET9, JJAZ1, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein

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