TTBK2 ELISA Kit

Catalog No. ABIN5659098

Product Details TTBK2 ELISA Kit

Target: TTBK2 (tau Tubulin Kinase 2 (TTBK2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Tau-Tubulin Kinase 2 (tTBK2). No significant cross-reactivity or interference between Tau-Tubulin Kinase 2 (tTBK2) and analogues was observed.

Sensitivity: 0.124 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tau-Tubulin Kinase 2 (tTBK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tau-Tubulin Kinase 2 (tTBK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tau-Tubulin Kinase 2 (tTBK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tau-Tubulin Kinase 2 (tTBK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tau-Tubulin Kinase 2 (tTBK2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tau-Tubulin Kinase 2 (tTBK2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TTBK2

Target: TTBK2 (tau Tubulin Kinase 2 (TTBK2))

Alternative Name: Tau-Tubulin Kinase 2 (TTBK2 Products)

Synonyms: ttbk ELISA Kit, MGC146331 ELISA Kit, 2610507N02Rik ELISA Kit, AI326283 ELISA Kit, B930008N24Rik ELISA Kit, TTK ELISA Kit, Ttbk ELISA Kit, Ttbk1 ELISA Kit, mKIAA0847 ELISA Kit, SCA11 ELISA Kit, TTBK ELISA Kit, tau tubulin kinase 2 ELISA Kit, TTBK2 ELISA Kit, ttbk2 ELISA Kit, Ttbk2 ELISA Kit

Background:

Gene Name: Tau-Tubulin Kinase 2

Gene Aliases: SCA11, Spinocerebellar Ataxia 11

Gene ID: 146057

UniProt: Q6IQ55