TERT ELISA Kit

Catalog No. ABIN5659110

Product Details TERT ELISA Kit

Target: TERT (Telomerase Reverse Transcriptase (TERT))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Telomerase Reverse Transcriptase (TERT). No significant cross-reactivity or interference between Telomerase Reverse Transcriptase (TERT) and analogues was observed.

Sensitivity: 13.7 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Telomerase Reverse Transcriptase (TERT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Telomerase Reverse Transcriptase (TERT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Telomerase Reverse Transcriptase (TERT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Telomerase Reverse Transcriptase (TERT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Telomerase Reverse Transcriptase (TERT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Telomerase Reverse Transcriptase (TERT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TERT

Target: TERT (Telomerase Reverse Transcriptase (TERT))

Alternative Name: Telomerase Reverse Transcriptase (TERT Products)

Synonyms: CMM9 ELISA Kit, DKCA2 ELISA Kit, DKCB4 ELISA Kit, EST2 ELISA Kit, PFBMFT1 ELISA Kit, TCS1 ELISA Kit, TP2 ELISA Kit, TRT ELISA Kit, hEST2 ELISA Kit, hTRT ELISA Kit, chTERT ELISA Kit, Tert ELISA Kit, ATTERT ELISA Kit, F5E19.190 ELISA Kit, F5E19_190 ELISA Kit, telomerase reverse transcriptase ELISA Kit, tert ELISA Kit, TR ELISA Kit, est2 ELISA Kit, tcs1 ELISA Kit, telomerase ELISA Kit, tert-A ELISA Kit, tp2 ELISA Kit, trt ELISA Kit, xTERT ELISA Kit, telomerase reverse transcriptase ELISA Kit, Telomerase Reverse Transcriptase ELISA Kit, Tertp ELISA Kit, Telomerase catalytic subunit ELISA Kit, telomerase reverse transcriptase L homeolog ELISA Kit, TERT ELISA Kit, trt-1 ELISA Kit, Tert ELISA Kit, tert ELISA Kit, CMU_003490 ELISA Kit, VDBG_04487 ELISA Kit, GL50803_16225 ELISA Kit, tert.L ELISA Kit

Background:

Gene Name: Telomerase Reverse Transcriptase

Gene Aliases: EST2, TCS1, TP2, TRT, hEST2, Telomerase catalytic subunit, Telomerase-associated protein 2

Gene ID: 301965

UniProt: Q673L6

Pathways: Telomere Maintenance