TNC ELISA Kit

Catalog No. ABIN5659114

Product Details TNC ELISA Kit

Target: TNC (Tenascin C (TNC))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Tenascin C (TNC). No significant cross-reactivity or interference between Tenascin C (TNC) and analogues was observed.

Sensitivity: 13.1 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tenascin C (TNC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tenascin C (TNC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tenascin C (TNC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tenascin C (TNC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tenascin C (TNC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tenascin C (TNC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TNC

Target: TNC (Tenascin C (TNC))

Alternative Name: Tenascin C (TNC Products)

Synonyms: tn ELISA Kit, 150-225 ELISA Kit, GMEM ELISA Kit, GP ELISA Kit, HXB ELISA Kit, JI ELISA Kit, TN ELISA Kit, TN-C ELISA Kit, AI528729 ELISA Kit, C130033P17Rik ELISA Kit, Hxb ELISA Kit, Ten ELISA Kit, cytotactin ELISA Kit, tenascin-C ELISA Kit, P230 ELISA Kit, tenc ELISA Kit, wu:fk04d02 ELISA Kit, tenascin C L homeolog ELISA Kit, tenascin C ELISA Kit, tnc.L ELISA Kit, TNC ELISA Kit, MCYG_00667 ELISA Kit, tnc ELISA Kit, Tnc ELISA Kit

Background:

Gene Name: Tenascin C

Gene Aliases: TN-C, HXB, TN, GMEM, JI, Myotendinous antigen, Neuronectin, GP 150-225, Cytotactin, Hexabrachion, Glioma-associated-extracellular matrix antigen

Gene ID: 3371

UniProt: P24821

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development