TPMT ELISA Kit

Catalog No. ABIN5659145

Product Details TPMT ELISA Kit

Target: TPMT (Thiopurine S-Methyltransferase (TPMT))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Thiopurine Methyltransferase (TPMT). No significant cross-reactivity or interference between Thiopurine Methyltransferase (TPMT) and analogues was observed.

Sensitivity: 0.127 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thiopurine Methyltransferase (TPMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thiopurine Methyltransferase (TPMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thiopurine Methyltransferase (TPMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thiopurine Methyltransferase (TPMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thiopurine Methyltransferase (TPMT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thiopurine Methyltransferase (TPMT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TPMT

Target: TPMT (Thiopurine S-Methyltransferase (TPMT))

Alternative Name: Thiopurine Methyltransferase (TPMT Products)

Synonyms: MGC84277 ELISA Kit, AW106912 ELISA Kit, NHLRC1 ELISA Kit, thiopurine S-methyltransferase L homeolog ELISA Kit, thiopurine S-methyltransferase, tandem duplicate 2 ELISA Kit, thiopurine S-methyltransferase TpmT ELISA Kit, thiopurine S-methyltransferase ELISA Kit, Thiopurine S-methyltransferase ELISA Kit, thiopurine methyltransferase ELISA Kit, tpmt.L ELISA Kit, tpmt.2 ELISA Kit, tpmT ELISA Kit, MCA0794 ELISA Kit, Pnap_4948 ELISA Kit, Hhal_1383 ELISA Kit, TK90_1044 ELISA Kit, Slit_1144 ELISA Kit, Tint_0842 ELISA Kit, Fbal_3334 ELISA Kit, Celly_3188 ELISA Kit, Weevi_1737 ELISA Kit, Psefu_2696 ELISA Kit, Metme_3860 ELISA Kit, Tpmt ELISA Kit, TPMT ELISA Kit

Background:

Gene Name: Thiopurine Methyltransferase

Gene Aliases: Thiopurine S Methyltransferase

Gene ID: 7172

UniProt: P51580