Thymopoietin ELISA Kit

Catalog No. ABIN5659214

Product Details Thymopoietin ELISA Kit

Target: Thymopoietin (TMPO)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.81 pg/mL - 500 pg/mL

Minimum Detection Limit: 7.81 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Thymopoietin (TMPO). No significant cross-reactivity or interference between Thymopoietin (TMPO) and analogues was observed.

Sensitivity: 12.9 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymopoietin (TMPO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymopoietin (TMPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymopoietin (TMPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thymopoietin (TMPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymopoietin (TMPO) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymopoietin (TMPO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Thymopoietin

Target: Thymopoietin (TMPO)

Alternative Name: Thymopoietin (TMPO Products)

Synonyms: LAP2 ELISA Kit, CMD1T ELISA Kit, LEMD4 ELISA Kit, PRO0868 ELISA Kit, TP ELISA Kit, lap2 ELISA Kit, LAP2beta ELISA Kit, 5630400D24Rik ELISA Kit, AI195756 ELISA Kit, AI606875 ELISA Kit, AW214352 ELISA Kit, AW547477 ELISA Kit, tmpo ELISA Kit, wu:fc26f12 ELISA Kit, wu:fd21f02 ELISA Kit, wu:fi29h05 ELISA Kit, thymopoietin ELISA Kit, thymopoietin S homeolog ELISA Kit, thymopoietin a ELISA Kit, TMPO ELISA Kit, Tmpo ELISA Kit, tmpo.S ELISA Kit, tmpoa ELISA Kit

Background:

Gene Name: Thymopoietin

Gene Aliases: TP, LAP2, CMD1T, LEMD4, TP5, Splenin, Lamina-Associated Polypeptide 2,Isoforms Beta/Gamma, LEM Domain Containing 4, Thymopoietin-related peptide isoforms beta/gamma

Gene ID: 7112

UniProt: P42167