Thymosin beta 10 ELISA Kit

Catalog No. ABIN5659215

Product Details Thymosin beta 10 ELISA Kit

Target: Thymosin beta 10 (TMSB10)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 24.69 ng/mL - 2000 ng/mL

Minimum Detection Limit: 24.69 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Thymosin Beta 10 (TMSb10). No significant cross-reactivity or interference between Thymosin Beta 10 (TMSb10) and analogues was observed.

Sensitivity: 13.52 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 h

Plate: Pre-coated

Protocol: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Thymosin Beta 10 (TMSb10) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Thymosin Beta 10 (TMSb10) and unlabeled Thymosin Beta 10 (TMSb10) (Standards or samples) with the pre-coated antibody specific to Thymosin Beta 10 (TMSb10). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Thymosin Beta 10 (TMSb10) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Thymosin Beta 10 (TMSb10) in the sample.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymosin Beta 10 (TMSb10) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymosin Beta 10 (TMSb10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Thymosin beta 10

Target: Thymosin beta 10 (TMSB10)

Alternative Name: Thymosin Beta 10 (TMSB10 Products)

Synonyms: TMSB10 ELISA Kit, DKFZp469K1916 ELISA Kit, MIG12 ELISA Kit, TB10 ELISA Kit, Ptmb10 ELISA Kit, THYb10 ELISA Kit, Tb10 ELISA Kit, thymosin beta 10 ELISA Kit, thymosin, beta 10 ELISA Kit, thymosin beta 4, Y-linked ELISA Kit, TMSB10 ELISA Kit, Tmsb10 ELISA Kit, TMSB4Y ELISA Kit

Background:

Gene Name: Thymosin Beta 10

Gene Aliases: MIG12, TB10, PTMB10

Pathways: Regulation of Actin Filament Polymerization, Maintenance of Protein Location