TSH receptor ELISA Kit

Catalog No. ABIN5659243

Product Details TSH receptor ELISA Kit

Target: TSH receptor (TSHR) (Thyroid Stimulating Hormone Receptor (TSHR))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Thyroid Stimulating Hormone Receptor (TSHR). No significant cross-reactivity or interference between Thyroid Stimulating Hormone Receptor (TSHR) and analogues was observed.

Sensitivity: 0.118 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroid Stimulating Hormone Receptor (TSHR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thyroid Stimulating Hormone Receptor (TSHR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyroid Stimulating Hormone Receptor (TSHR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroid Stimulating Hormone Receptor (TSHR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroid Stimulating Hormone Receptor (TSHR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroid Stimulating Hormone Receptor (TSHR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TSH receptor

Target: TSH receptor (TSHR) (Thyroid Stimulating Hormone Receptor (TSHR))

Alternative Name: Thyroid Stimulating Hormone Receptor (TSHR Products)

Synonyms: CHNG1 ELISA Kit, LGR3 ELISA Kit, hTSHR-I ELISA Kit, AI481368 ELISA Kit, hypothroid ELISA Kit, hyt ELISA Kit, pet ELISA Kit, TSHRA ELISA Kit, TSH-R ELISA Kit, thyroid stimulating hormone receptor ELISA Kit, tshr ELISA Kit, TSHR ELISA Kit, Tshr ELISA Kit

Background:

Gene Name: Thyroid Stimulating Hormone Receptor

Gene Aliases: LGR3, HTSHR-I, Thyrotropin Receptor, TSH Receptor

Gene ID: 25360

UniProt: P21463

Pathways: Thyroid Hormone Synthesis