Topoisomerase I ELISA Kit

Catalog No. ABIN5659376

Product Details Topoisomerase I ELISA Kit

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Topoisomerase I (TOP1). No significant cross-reactivity or interference between Topoisomerase I (TOP1) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Topoisomerase I (TOP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Topoisomerase I (TOP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Topoisomerase I (TOP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Topoisomerase I (TOP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Topoisomerase I (TOP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Topoisomerase I (TOP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: Topoisomerase I (TOP1 Products)

Synonyms: CG6146 ELISA Kit, Dmel\\CG6146 ELISA Kit, TOP1 ELISA Kit, TopI ELISA Kit, Topo I ELISA Kit, TopoI ELISA Kit, dTopoI ELISA Kit, l(1)G0134 ELISA Kit, l(1)G0201 ELISA Kit, l(1)G0229 ELISA Kit, l(1)G0278 ELISA Kit, l(1)top1 ELISA Kit, top1 ELISA Kit, topo I ELISA Kit, topoI ELISA Kit, DNA topoisomerase 1 beta ELISA Kit, DNA topoisomerase I alpha ELISA Kit, MGO1 ELISA Kit, MGOUN 1 ELISA Kit, MTE17.1 ELISA Kit, MTE17_1 ELISA Kit, TOP1BETA ELISA Kit, TOPOISOMERASE 1 ELISA Kit, TOPI ELISA Kit, AI467334 ELISA Kit, D130064I21Rik ELISA Kit, Top-1 ELISA Kit, Ab2-086 ELISA Kit, topi ELISA Kit, Topoisomerase 1 ELISA Kit, DNA topoisomerase I ELISA Kit, DNA topoisomerase I alpha ELISA Kit, DNA topoisomerase 1 ELISA Kit, topoisomerase (DNA) I ELISA Kit, DNA topoisomerase I, gene 1 L homeolog ELISA Kit, Top1 ELISA Kit, top1 ELISA Kit, topA ELISA Kit, ZMO1193 ELISA Kit, MRAD2831_RS63095 ELISA Kit, Lferr_0016 ELISA Kit, PC1_2015 ELISA Kit, Za10_0136 ELISA Kit, Gbro_0575 ELISA Kit, Dd586_2108 ELISA Kit, Kvar_3044 ELISA Kit, Alvin_2069 ELISA Kit, Dacet_0849 ELISA Kit, Mrub_1615 ELISA Kit, Arnit_2962 ELISA Kit, Ndas_4953 ELISA Kit, Trad_2377 ELISA Kit, Acear_1653 ELISA Kit, Mahau_0844 ELISA Kit, Mesop_4109 ELISA Kit, Thein_1586 ELISA Kit, Theth_0241 ELISA Kit, TOP1ALPHA ELISA Kit, top-1 ELISA Kit, TOP1 ELISA Kit, top1.1.L ELISA Kit

Target Type: Viral Protein

Background:

Gene Name: Topoisomerase I

Gene Aliases: TOPI, DNA Topoisomerase 1

Gene ID: 7150

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance