TWF1 ELISA Kit

Catalog No. ABIN5659700

Product Details TWF1 ELISA Kit

Target: TWF1 (Twinfilin, Actin-Binding Protein 1 (TWF1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Twinfilin 1 (TWF1). No significant cross-reactivity or interference between Twinfilin 1 (TWF1) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Twinfilin 1 (TWF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Twinfilin 1 (TWF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Twinfilin 1 (TWF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Twinfilin 1 (TWF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Twinfilin 1 (TWF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Twinfilin 1 (TWF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TWF1

Target: TWF1 (Twinfilin, Actin-Binding Protein 1 (TWF1))

Alternative Name: Twinfilin 1 (TWF1 Products)

Synonyms: A6 ELISA Kit, PTK9 ELISA Kit, Ptk9 ELISA Kit, twinfilin ELISA Kit, ptk9 ELISA Kit, twf1 ELISA Kit, wu:fd02b03 ELISA Kit, zgc:65922 ELISA Kit, DKFZp469O1925 ELISA Kit, CG3771 ELISA Kit, Dmel\\CG3771 ELISA Kit, EG:9D2.3 ELISA Kit, zgc:92472 ELISA Kit, twinfilin actin binding protein 1 ELISA Kit, twinfilin actin-binding protein 1a ELISA Kit, twinfilin actin binding protein 1 S homeolog ELISA Kit, twinfilin-1 ELISA Kit, hypothetical protein ELISA Kit, Twinfilin-1 ELISA Kit, twinfilin actin-binding protein 1 ELISA Kit, CG3771 gene product from transcript CG3771-RC ELISA Kit, twinfilin actin-binding protein 1b ELISA Kit, TWF1 ELISA Kit, Twf1 ELISA Kit, twf1a ELISA Kit, twf1.S ELISA Kit, PTRG_09288 ELISA Kit, PGTG_01788 ELISA Kit, Tsp_02530 ELISA Kit, twf1 ELISA Kit, a6 ELISA Kit, twf1b ELISA Kit

Background:

Gene Name: Twinfilin 1

Gene Aliases: A6, PTK9, Actin-Binding Protein,Homolog 1, Protein Tyrosine Kinase 9

Gene ID: 5756

UniProt: Q12792

Pathways: Regulation of Actin Filament Polymerization, Maintenance of Protein Location