UCHL1 CLIA Kit

Catalog No. ABIN5659729

Product Details UCHL1 CLIA Kit

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivity: Rat

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 234.38 pg/mL - 15000 pg/mL

Minimum Detection Limit: 234.38 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). No significant cross-reactivity or interference between Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) and analogues was observed.

Sensitivity: 7.3 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name: Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1 Products)

Synonyms: PARK5 CLIA Kit, PGP 9.5 CLIA Kit, PGP9.5 CLIA Kit, PGP95 CLIA Kit, Uch-L1 CLIA Kit, UCH-L1 CLIA Kit, UCHL1 CLIA Kit, cb358 CLIA Kit, wu:fc55h08 CLIA Kit, park5 CLIA Kit, pgp9.5 CLIA Kit, uch-l1 CLIA Kit, MGC132191 CLIA Kit, uchl1 CLIA Kit, AW822034 CLIA Kit, C88048 CLIA Kit, R75593 CLIA Kit, UCHL-1 CLIA Kit, gad CLIA Kit, ubiquitin C-terminal hydrolase L1 CLIA Kit, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) CLIA Kit, ubiquitin C-terminal hydrolase L1 L homeolog CLIA Kit, ubiquitin carboxyl-terminal esterase L1 CLIA Kit, ubiquitin carboxy-terminal hydrolase L1 CLIA Kit, UCHL1 CLIA Kit, uchl1 CLIA Kit, uchl1.L CLIA Kit, LOC100304750 CLIA Kit, Uchl1 CLIA Kit

Background:

Gene Name: Ubiquitin Carboxyl Terminal Hydrolase L1

Gene Aliases: PARK5, PGP9.5, Uch-L1, Neuron cytoplasmic protein 9.5, Ubiquitin thioesterase L1, Ubiquitin carboxyl-terminal hydrolase isozyme L1

Gene ID: 29545

UniProt: Q00981

Pathways: Feeding Behaviour