USP14 ELISA Kit

Catalog No. ABIN5659744

Product Details USP14 ELISA Kit

Target: USP14 (Ubiquitin Specific Peptidase 14 (USP14))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ubiquitin Specific Peptidase 14 (USP14). No significant cross-reactivity or interference between Ubiquitin Specific Peptidase 14 (USP14) and analogues was observed.

Sensitivity: 0.108 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Specific Peptidase 14 (USP14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Specific Peptidase 14 (USP14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Specific Peptidase 14 (USP14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin Specific Peptidase 14 (USP14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Specific Peptidase 14 (USP14) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Specific Peptidase 14 (USP14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for USP14

Target: USP14 (Ubiquitin Specific Peptidase 14 (USP14))

Alternative Name: Ubiquitin Specific Peptidase 14 (USP14 Products)

Synonyms: TGT ELISA Kit, DDBDRAFT_0202821 ELISA Kit, DDBDRAFT_0237695 ELISA Kit, DDB_0202821 ELISA Kit, DDB_0237695 ELISA Kit, 2610005K12Rik ELISA Kit, 2610037B11Rik ELISA Kit, AW107924 ELISA Kit, C78769 ELISA Kit, ax ELISA Kit, zgc:55949 ELISA Kit, wu:fk63d09 ELISA Kit, MGC81945 ELISA Kit, tgt ELISA Kit, MGC69343 ELISA Kit, USP14 ELISA Kit, ubiquitin specific peptidase 14 ELISA Kit, peptidase C19 family protein ELISA Kit, ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) ELISA Kit, ubiquitin specific protease 14 ELISA Kit, ubiquitin specific peptidase 14 S homeolog ELISA Kit, universal stress protein ELISA Kit, Ubiquitin carboxyl-terminal hydrolase 14 ELISA Kit, USP14 ELISA Kit, usp14 ELISA Kit, Usp14 ELISA Kit, Bm1_28765 ELISA Kit, usp14.S ELISA Kit, RR_RS06480 ELISA Kit, usp-14 ELISA Kit

Background:

Gene Name: Ubiquitin Specific Peptidase 14

Gene Aliases: TGT, tRNA-Guanine Transglycosylase, Ubiquitin Specific Protease 14, Deubiquitinating enzyme 14, Ubiquitin carboxyl-terminal hydrolase 14

Gene ID: 9097

UniProt: P54578