K-RAS ELISA Kit

Catalog No. ABIN5659809

Product Details K-RAS ELISA Kit

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS). No significant cross-reactivity or interference between V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) and analogues was observed.

Sensitivity: 0.125 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for K-RAS

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Alternative Name: V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS Products)

Synonyms: wu:fa04e08 ELISA Kit, wu:fc14b12 ELISA Kit, wu:fc23g10 ELISA Kit, wu:fj89d12 ELISA Kit, zgc:85725 ELISA Kit, Kras2 ELISA Kit, c-Ki-ras ELISA Kit, p21 ELISA Kit, C-K-RAS ELISA Kit, CFC2 ELISA Kit, K-RAS2A ELISA Kit, K-RAS2B ELISA Kit, K-RAS4A ELISA Kit, K-RAS4B ELISA Kit, KI-RAS ELISA Kit, KRAS1 ELISA Kit, KRAS2 ELISA Kit, NS ELISA Kit, NS3 ELISA Kit, RASK2 ELISA Kit, AI929937 ELISA Kit, K-ras ELISA Kit, Ki-ras ELISA Kit, Kras-2 ELISA Kit, p21B ELISA Kit, ras ELISA Kit, k-ras ELISA Kit, kras ELISA Kit, kras-a ELISA Kit, kras-b ELISA Kit, rask2 ELISA Kit, K-RAS ELISA Kit, p21ras ELISA Kit, KRAS proto-oncogene, GTPase ELISA Kit, v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog ELISA Kit, Kirsten rat sarcoma viral oncogene homolog ELISA Kit, semicolonial-7 ELISA Kit, Ras family protein ELISA Kit, GTPase KRas ELISA Kit, Kirsten rat sarcoma viral oncogene homolog S homeolog ELISA Kit, KRAS ELISA Kit, kras ELISA Kit, Kras ELISA Kit, smco-7 ELISA Kit, PGTG_03066 ELISA Kit, Tsp_02666 ELISA Kit, Tsp_01642 ELISA Kit, rask ELISA Kit, kras.S ELISA Kit

Target Type: Viral Protein

Background:

Gene Name: V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog

Gene Aliases: C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS3, RASK2

Gene ID: 3845

UniProt: P01116