Vinculin ELISA Kit

Catalog No. ABIN5659937

Product Details Vinculin ELISA Kit

Target: Vinculin (VCL)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Vinculin (VCL). No significant cross-reactivity or interference between Vinculin (VCL) and analogues was observed.

Sensitivity: 0.128 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vinculin (VCL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vinculin (VCL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vinculin (VCL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vinculin (VCL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vinculin (VCL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vinculin (VCL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: Vinculin (VCL Products)

Synonyms: CMD1W ELISA Kit, CMH15 ELISA Kit, MV ELISA Kit, MVCL ELISA Kit, VCL ELISA Kit, im:6902790 ELISA Kit, hm:zehn2160 ELISA Kit, DDBDRAFT_0186762 ELISA Kit, DDBDRAFT_0232320 ELISA Kit, DDB_0186762 ELISA Kit, DDB_0232320 ELISA Kit, 9430097D22 ELISA Kit, AA571387 ELISA Kit, AI462105 ELISA Kit, AW545629 ELISA Kit, CG3299 ELISA Kit, CT11081 ELISA Kit, Dmel\\CG3299 ELISA Kit, Dmvincp ELISA Kit, EG:103B4.1 ELISA Kit, Vin ELISA Kit, Vin2EF ELISA Kit, vinc ELISA Kit, LOC100228062 ELISA Kit, vcl-A ELISA Kit, vinculin ELISA Kit, vinculin a ELISA Kit, vinculin A ELISA Kit, Vinculin ELISA Kit, vinculin S homeolog ELISA Kit, VCL ELISA Kit, vcla ELISA Kit, vinA ELISA Kit, Vcl ELISA Kit, Vinc ELISA Kit, CpipJ_CPIJ013873 ELISA Kit, Bm1_37935 ELISA Kit, vcl ELISA Kit, vcl.S ELISA Kit

Background:

Gene Name: Vinculin

Gene Aliases: MVCL, Metavinculin

Gene ID: 22330

UniProt: Q64727

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2