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WWP2 ELISA Kit

WWP2 Reactivity: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Lysate, Tissue Homogenate
Catalog No. ABIN5660045
  • Target See all WWP2 ELISA Kits
    WWP2 (WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2))
    Reactivity
    • 2
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.781 ng/mL - 50 ng/mL
    Minimum Detection Limit
    0.781 ng/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2). No significant cross-reactivity or interference between WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) and analogues was observed.
    Sensitivity
    0.27 ng/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all WWP2 ELISA Kits
    WWP2 (WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2))
    Alternative Name
    WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2 Products)
    Synonyms
    aip2 ELISA Kit, wwp2-like ELISA Kit, id:ibd1121 ELISA Kit, wu:fo87e10 ELISA Kit, zgc:154036 ELISA Kit, AIP2 ELISA Kit, WWp2-like ELISA Kit, 1300010O06Rik ELISA Kit, AA690238 ELISA Kit, AW554328 ELISA Kit, WW domain containing E3 ubiquitin protein ligase 2 ELISA Kit, WWP2 ELISA Kit, wwp2 ELISA Kit, Wwp2 ELISA Kit
    Background

    Gene Name: WW Domain Containing E3 Ubiquitin Protein Ligase 2

    Gene Aliases: AIP2, WWp2-like, Atrophin-1-interacting protein 2, NEDD4-like E3 ubiquitin-protein ligase WWP2

    Gene ID
    11060
    UniProt
    O00308
    Pathways
    Negative Regulation of Transporter Activity
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