XBP1 ELISA Kit

Catalog No. ABIN5660050

Product Details XBP1 ELISA Kit

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of X-Box Binding Protein 1 (XBP1). No significant cross-reactivity or interference between X-Box Binding Protein 1 (XBP1) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to X-Box Binding Protein 1 (XBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to X-Box Binding Protein 1 (XBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain X-Box Binding Protein 1 (XBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of X-Box Binding Protein 1 (XBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level X-Box Binding Protein 1 (XBP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level X-Box Binding Protein 1 (XBP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Alternative Name: X-Box Binding Protein 1 (XBP1 Products)

Synonyms: treb5 ELISA Kit, xXBP1 ELISA Kit, xbp2 ELISA Kit, XBP1P1 ELISA Kit, XBPP1 ELISA Kit, xbp1 ELISA Kit, TREB5 ELISA Kit, XBP-1 ELISA Kit, XBP2 ELISA Kit, D11Ertd39e ELISA Kit, TREB-5 ELISA Kit, HTF ELISA Kit, cb918 ELISA Kit, id:ibd1195 ELISA Kit, trebf ELISA Kit, wu:fb02h05 ELISA Kit, wu:fb63f10 ELISA Kit, xbp1a ELISA Kit, xbp1b ELISA Kit, X-box binding protein 1 ELISA Kit, X-box binding protein 1 L homeolog ELISA Kit, xbp1 ELISA Kit, XBP1 ELISA Kit, Xbp1 ELISA Kit, xbp1.L ELISA Kit

Background:

Gene Name: X-Box Binding Protein 1

Gene Aliases: TREB5, XBP2, Tax-responsive element-binding protein 5

Pathways: ER-Nucleus Signaling, Unfolded Protein Response