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ADORA1 ELISA Kit

ADORA1 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Catalog No. ABIN5674664
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    ADORA1 (Adenosine A1 Receptor (ADORA1))
    Reactivity
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156 ng/mL - 10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensitivity
    0061 ng/mL
    Components
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target See all ADORA1 ELISA Kits
    ADORA1 (Adenosine A1 Receptor (ADORA1))
    Abstract
    ADORA1 Products
    Synonyms
    adora1-A ELISA Kit, a1R ELISA Kit, ADORA1 ELISA Kit, LOC606743 ELISA Kit, LOC100222054 ELISA Kit, RDC7 ELISA Kit, A1-AR ELISA Kit, A1AR ELISA Kit, A1R ELISA Kit, AA1R ELISA Kit, AI848715 ELISA Kit, BB176431 ELISA Kit, Ri ELISA Kit, adenosine A1 receptor L homeolog ELISA Kit, adenosine A1 receptor ELISA Kit, adenosine receptor A1 ELISA Kit, adora1.L ELISA Kit, ADORA1 ELISA Kit, adora1 ELISA Kit, LOC100222054 ELISA Kit, Adora1 ELISA Kit
    Background
    Alternative name: RDC7
    Gene ID
    134
    UniProt
    P30542
    Pathways
    EGFR Signaling Pathway, Negative Regulation of Hormone Secretion, Synaptic Membrane
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