Apelin Receptor ELISA Kit

Catalog No. ABIN5674666

Product Details Apelin Receptor ELISA Kit

Target: Apelin Receptor (APLNR)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.625 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.625 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 5.7 pg/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for Apelin Receptor

Target: Apelin Receptor (APLNR)

Abstract: APLNR Products

Synonyms: AGTRL1 ELISA Kit, APJ ELISA Kit, APJR ELISA Kit, HG11 ELISA Kit, Agtrl1 ELISA Kit, msr/apj ELISA Kit, Apj ELISA Kit, X-msr ELISA Kit, Xangio1 ELISA Kit, Xmsr ELISA Kit, agtrl1 ELISA Kit, angio1 ELISA Kit, apj ELISA Kit, apjr ELISA Kit, hg11 ELISA Kit, msr ELISA Kit, APLNR ELISA Kit, DKFZp469C1532 ELISA Kit, agtrl1-B ELISA Kit, agtrl1b ELISA Kit, Agtrl1a ELISA Kit, grn ELISA Kit, zgc:114063 ELISA Kit, APJa ELISA Kit, XAngio1 ELISA Kit, agtrl1-A ELISA Kit, agtrl1a ELISA Kit, aplnr-a ELISA Kit, aplnr-b ELISA Kit, x-MSR ELISA Kit, xAngio1 ELISA Kit, APLNR1 ELISA Kit, apelin receptor ELISA Kit, apelin receptor S homeolog ELISA Kit, apelin receptor b ELISA Kit, apelin receptor L homeolog ELISA Kit, APLNR ELISA Kit, Aplnr ELISA Kit, aplnr ELISA Kit, aplnr.S ELISA Kit, aplnrb ELISA Kit, aplnr.L ELISA Kit

Background: Alternative name: AGTRL1, HG11, APJR, APJ, APJ Receptor, Angiotensin II Receptor-Like 1, Angiotensin receptor-like 1

Gene ID: 187

UniProt: P35414