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Apelin Receptor ELISA Kit

APLNR Reactivity: Mouse Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Tissue Homogenate
Catalog No. ABIN5674667
  • Target See all Apelin Receptor (APLNR) ELISA Kits
    Apelin Receptor (APLNR)
    Reactivity
    • 2
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.625 pg/mL - 1000 pg/mL
    Minimum Detection Limit
    15.625 pg/mL
    Application
    ELISA
    Sample Type
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensitivity
    5.9 pg/mL
    Components
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target See all Apelin Receptor (APLNR) ELISA Kits
    Apelin Receptor (APLNR)
    Abstract
    APLNR Products
    Synonyms
    AGTRL1 ELISA Kit, APJ ELISA Kit, APJR ELISA Kit, HG11 ELISA Kit, Agtrl1 ELISA Kit, msr/apj ELISA Kit, Apj ELISA Kit, X-msr ELISA Kit, Xangio1 ELISA Kit, Xmsr ELISA Kit, agtrl1 ELISA Kit, angio1 ELISA Kit, apj ELISA Kit, apjr ELISA Kit, hg11 ELISA Kit, msr ELISA Kit, APLNR ELISA Kit, DKFZp469C1532 ELISA Kit, agtrl1-B ELISA Kit, agtrl1b ELISA Kit, Agtrl1a ELISA Kit, grn ELISA Kit, zgc:114063 ELISA Kit, APJa ELISA Kit, XAngio1 ELISA Kit, agtrl1-A ELISA Kit, agtrl1a ELISA Kit, aplnr-a ELISA Kit, aplnr-b ELISA Kit, x-MSR ELISA Kit, xAngio1 ELISA Kit, APLNR1 ELISA Kit, apelin receptor ELISA Kit, apelin receptor S homeolog ELISA Kit, apelin receptor b ELISA Kit, apelin receptor L homeolog ELISA Kit, APLNR ELISA Kit, Aplnr ELISA Kit, aplnr ELISA Kit, aplnr.S ELISA Kit, aplnrb ELISA Kit, aplnr.L ELISA Kit
    Background
    Alternative name: AGTRL1, HG11, APJR, APJ, APJ Receptor, Angiotensin II Receptor-Like 1, Angiotensin receptor-like 1
    Gene ID
    23796
    UniProt
    Q9WV08
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