C9 ELISA Kit

Catalog No. ABIN5674670

Product Details C9 ELISA Kit

Target: C9 (Complement Component C9 (C9))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.468 ng/mL - 30 ng/mL

Minimum Detection Limit: 0.468 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0.19 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for C9

Target: C9 (Complement Component C9 (C9))

Alternative Name: Complement Component 9 (C9) (C9 Products)

Synonyms: LOC100037951 ELISA Kit, LOC100136130 ELISA Kit, c9 ELISA Kit, wu:fb60b05 ELISA Kit, wu:fd50e04 ELISA Kit, zgc:112272 ELISA Kit, complement C9 ELISA Kit, complement component 9 ELISA Kit, complement component 9 L homeolog ELISA Kit, C9 ELISA Kit, c9 ELISA Kit, c9.L ELISA Kit

Background: Alternative name: Complement component C9, C9

Gene ID: 12279

UniProt: P06683

Pathways: Complement System