PLP1 ELISA Kit

Catalog No. ABIN5674700

Product Details PLP1 ELISA Kit

Target: PLP1 (Proteolipid Protein 1 (PLP1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 26 pg/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for PLP1

Target: PLP1 (Proteolipid Protein 1 (PLP1))

Alternative Name: Proteolipid Protein 1, Myelin (PLP1) (PLP1 Products)

Synonyms: GPM6C ELISA Kit, HLD1 ELISA Kit, MMPL ELISA Kit, PLP ELISA Kit, PLP/DM20 ELISA Kit, PMD ELISA Kit, SPG2 ELISA Kit, Plp ELISA Kit, PLP1 ELISA Kit, plp1 ELISA Kit, DKFZp459O081 ELISA Kit, DKFZp459O113 ELISA Kit, DM20 ELISA Kit, jimpy ELISA Kit, jp ELISA Kit, msd ELISA Kit, rsh ELISA Kit, plp ELISA Kit, hld1 ELISA Kit, mmpl ELISA Kit, plp1a ELISA Kit, pmd ELISA Kit, spg2 ELISA Kit, DMalpha2c ELISA Kit, wu:fc27f01 ELISA Kit, wu:fj36d03 ELISA Kit, wu:fj42d08 ELISA Kit, zgc:110499 ELISA Kit, PLP-B ELISA Kit, plp1-a ELISA Kit, plp1-b ELISA Kit, plp1b ELISA Kit, proteolipid protein 1 ELISA Kit, proteolipid protein (myelin) 1 ELISA Kit, myelin proteolipid protein ELISA Kit, proteolipid protein 1 L homeolog ELISA Kit, proteolipid protein 1a ELISA Kit, proteolipid protein 1 S homeolog ELISA Kit, PLP1 ELISA Kit, Plp1 ELISA Kit, plp1 ELISA Kit, Tsp_11640 ELISA Kit, plp ELISA Kit, plp1.L ELISA Kit, plp1a ELISA Kit, plp1.S ELISA Kit

Background: Alternative name: MMPL, PLP/DM20, PMD, SPG2, Pelizaeus-Merzbacher Disease, Spastic Paraplegia 2,Uncomplicated, Myelin proteolipid protein, Lipophilin

Gene ID: 24943

UniProt: P60203