MAT1A ELISA Kit

Catalog No. ABIN5674721

Product Details MAT1A ELISA Kit

Target: MAT1A (Methionine Adenosyltransferase I, alpha (MAT1A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 40 ng/mL

Minimum Detection Limit: 0.625 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0255 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for MAT1A

Target: MAT1A (Methionine Adenosyltransferase I, alpha (MAT1A))

Alternative Name: Methionine Adenosyltransferase I Alpha (MAT1a) (MAT1A Products)

Synonyms: MAT ELISA Kit, MATA1 ELISA Kit, SAMS ELISA Kit, SAMS1 ELISA Kit, AdoMet ELISA Kit, SADE ELISA Kit, SAS ELISA Kit, AI046368 ELISA Kit, Ams ELISA Kit, wu:fi35e01 ELISA Kit, zgc:55442 ELISA Kit, methionine adenosyltransferase 1A ELISA Kit, methionine adenosyltransferase I, alpha ELISA Kit, MAT1A ELISA Kit, Mat1a ELISA Kit, mat1a ELISA Kit

Background: Alternative name: MAT, SAMS, MATA1, SAMS1, AMS1, S-Adenosylmethionine Synthetase, S-adenosylmethionine synthase isoform type-1

Gene ID: 4143

UniProt: Q00266

Pathways: Mitotic G1-G1/S Phases, M Phase, Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process