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ADCY10 ELISA Kit

ADCY10 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
Catalog No. ABIN5674728
  • Target See all ADCY10 ELISA Kits
    ADCY10 (Adenylate Cyclase 10 (Soluble) (ADCY10))
    Reactivity
    • 2
    • 2
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156 ng/mL - 10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Sample Type
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensitivity
    0051 ng/mL
    Components
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target See all ADCY10 ELISA Kits
    ADCY10 (Adenylate Cyclase 10 (Soluble) (ADCY10))
    Alternative Name
    Adenylate Cyclase 10, Soluble (ADCY10) (ADCY10 Products)
    Synonyms
    HCA2 ELISA Kit, RP1-313L4.2 ELISA Kit, SAC ELISA Kit, SACI ELISA Kit, Sacy ELISA Kit, Sac ELISA Kit, 4930431D04Rik ELISA Kit, 4931412F17 ELISA Kit, sAC ELISA Kit, ADCY10 ELISA Kit, SACY ELISA Kit, adenylate cyclase 10 ELISA Kit, adenylate cyclase 10 (soluble) ELISA Kit, ADCY10 ELISA Kit, Adcy10 ELISA Kit
    Background
    Alternative name: SAC, HCA2, SACI, Sacy, Testicular soluble adenylyl cyclase, Hypercalciuria,Absorptive 2, AH-related protein, Germ cell soluble adenylyl cyclase
    Gene ID
    55811
    UniProt
    Q96PN6
    Pathways
    cAMP Metabolic Process
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