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VLDLR ELISA Kit

VLDLR Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Tissue Homogenate
Catalog No. ABIN5674735
  • Target See all VLDLR ELISA Kits
    VLDLR (Very Low Density Lipoprotein Receptor (VLDLR))
    Reactivity
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    1.56 ng/mL - 100 ng/mL
    Minimum Detection Limit
    1.56 ng/mL
    Application
    ELISA
    Sample Type
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensitivity
    0.53 ng/mL
    Components
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target See all VLDLR ELISA Kits
    VLDLR (Very Low Density Lipoprotein Receptor (VLDLR))
    Abstract
    VLDLR Products
    Synonyms
    CARMQ1 ELISA Kit, CHRMQ1 ELISA Kit, VLDLRCH ELISA Kit, AA408956 ELISA Kit, AI451093 ELISA Kit, AW047288 ELISA Kit, vldlr ELISA Kit, very low density lipoprotein receptor ELISA Kit, very low density lipoprotein receptor S homeolog ELISA Kit, VLDLR ELISA Kit, Vldlr ELISA Kit, vldlr.S ELISA Kit
    Background
    Alternative name: VLDL-R
    Gene ID
    22359
    UniProt
    P98156
    Pathways
    Cellular Response to Molecule of Bacterial Origin
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