FBLIM1 ELISA Kit
FBLIM1
Reactivity: Mouse
Colorimetric
Sandwich ELISA
0.156 ng/mL - 10 ng/mL
Plasma, Serum, Tissue Homogenate
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- Target See all FBLIM1 ELISA Kits
- FBLIM1 (Filamin Binding LIM Protein 1 (FBLIM1))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.156 ng/mL - 10 ng/mL
- Minimum Detection Limit
- 0.156 ng/mL
- Application
- ELISA
- Sample Type
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Sensitivity
- 0053 ng/mL
- Components
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Material not included
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
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Filamin Binding LIM Protein 1 (FBLIM1) ELISA Kit
FBLIM1 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Assay Time
- 1 - 4.5 h
- Plate
- Pre-coated
- Protocol
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Storage
- 4 °C
- Expiry Date
- 12 months
-
- Target See all FBLIM1 ELISA Kits
- FBLIM1 (Filamin Binding LIM Protein 1 (FBLIM1))
- Abstract
- FBLIM1 Products
- Synonyms
- FBLIM1 ELISA Kit, CAL ELISA Kit, FBLP-1 ELISA Kit, FBLP1 ELISA Kit, 2410043F08Rik ELISA Kit, Cal ELISA Kit, Fblp1 ELISA Kit, Gt10 ELISA Kit, migfilin ELISA Kit, migfilin(s) ELISA Kit, filamin binding LIM protein 1 ELISA Kit, filamin binding LIM protein 1 S homeolog ELISA Kit, FBLIM1 ELISA Kit, fblim1.S ELISA Kit, Fblim1 ELISA Kit
- Background
- Alternative name: CAL, FBLP-1, FBLP1, Migfilin, Mitogen-inducible 2-interacting protein
- Gene ID
- 74202
- UniProt
- Q71FD7
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