GBA ELISA Kit

Catalog No. ABIN5674742

Product Details GBA ELISA Kit

Target: GBA (Glucosidase, Beta, Acid (GBA))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0064 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for GBA

Target: GBA (Glucosidase, Beta, Acid (GBA))

Alternative Name: Glucosidase Beta, Acid (GbA) (GBA Products)

Synonyms: GBA1 ELISA Kit, GCB ELISA Kit, GLUC ELISA Kit, BETA-GLUCOSIDASE ELISA Kit, T12J13.8 ELISA Kit, T12J13_8 ELISA Kit, beta glucosidase 25 ELISA Kit, PSPTO3318 ELISA Kit, PSPTO4290 ELISA Kit, GC ELISA Kit, GCase ELISA Kit, betaGC ELISA Kit, glucosylceramidase beta ELISA Kit, beta glucosidase 25 ELISA Kit, beta-glucosidase ELISA Kit, Beta-glucosidase ELISA Kit, glucosidase, beta, acid ELISA Kit, glucosylceramidase ELISA Kit, bglX-2 ELISA Kit, GBA ELISA Kit, BGLU25 ELISA Kit, PSPTO_3318 ELISA Kit, bglX ELISA Kit, bglA4 ELISA Kit, Gba ELISA Kit, LOC100399524 ELISA Kit, bglA.2 ELISA Kit, bglX-2 ELISA Kit

Background: Alternative name: GBA1, GCB, GLUC, Imiglucerase, Alglucerase, Beta-glucocerebrosidase, Glucosylceramidase, β-Glucosidase, D-Glucosyl-N-Acylsphingosine Glucohydrolase

Gene ID: 14466

UniProt: P17439

Pathways: Cellular Glucan Metabolic Process