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CCR2 ELISA Kit

CCR2 Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5674764
  • Target See all CCR2 ELISA Kits
    CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))
    Reactivity
    • 3
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156 ng/mL - 10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensitivity
    0061 ng/mL
    Components
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target See all CCR2 ELISA Kits
    CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))
    Alternative Name
    Chemokine C-C-Motif Receptor 2 (CCR2) (CCR2 Products)
    Synonyms
    CC-CKR-2 ELISA Kit, CCR-2 ELISA Kit, CCR2A ELISA Kit, CCR2B ELISA Kit, CD192 ELISA Kit, CKR2 ELISA Kit, CKR2A ELISA Kit, CKR2B ELISA Kit, CMKBR2 ELISA Kit, MCP-1-R ELISA Kit, ACKR5 ELISA Kit, CKRX ELISA Kit, CRAM ELISA Kit, CRAM-A ELISA Kit, CRAM-B ELISA Kit, HCR ELISA Kit, Cc-ckr-2 ELISA Kit, Ccr2a ELISA Kit, Ccr2b ELISA Kit, Ckr2 ELISA Kit, Ckr2a ELISA Kit, Ckr2b ELISA Kit, Cmkbr2 ELISA Kit, mJe-r ELISA Kit, C-C motif chemokine receptor 2 ELISA Kit, C-C motif chemokine receptor like 2 ELISA Kit, chemokine (C-C motif) receptor 2 ELISA Kit, CCR2 ELISA Kit, CCRL2 ELISA Kit, Ccr2 ELISA Kit, LOC484790 ELISA Kit
    Background
    Alternative name: CD192, CC-CKR2, CCR2A, C-C CKR-2, CCR2B, CKR2-A, CKR2-B, CMKBR2, MCP1-R, Monocyte chemoattractant protein 1 receptor
    Gene ID
    12772
    UniProt
    P51683
    Pathways
    cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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