HTR2C ELISA Kit
HTR2C
Reactivity: Mouse
Colorimetric
Sandwich ELISA
78.125 pg/mL - 5000 pg/mL
Tissue Homogenate
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- Target See all HTR2C ELISA Kits
- HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 78.125 pg/mL - 5000 pg/mL
- Minimum Detection Limit
- 78.125 pg/mL
- Application
- ELISA
- Sample Type
- Tissue Homogenate
- Analytical Method
- Quantitative
- Sensitivity
- 26.6 pg/mL
- Components
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Material not included
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product HTR2C ELISA Kit
-
5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C) ELISA Kit
HTR2C Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Assay Time
- 1 - 4.5 h
- Plate
- Pre-coated
- Protocol
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Storage
- 4 °C
- Expiry Date
- 12 months
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- Target See all HTR2C ELISA Kits
- HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))
- Alternative Name
- 5-Hydroxytryptamine Receptor 2C (HTR2C) (HTR2C Products)
- Background
- Alternative name: 5-HT2C, HTR1C, Serotonin Receptor 2C, 5-hydroxytryptamine receptor 1C
- Gene ID
- 15560
- UniProt
- P34968
- Pathways
- Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour
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