ALDOA ELISA Kit

Catalog No. ABIN5674804

Product Details ALDOA ELISA Kit

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.125 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.125 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 34.5 pg/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for ALDOA

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Alternative Name: Aldolase A, Fructose Bisphosphate (ALDOA) (ALDOA Products)

Synonyms: ALDA ELISA Kit, GSD12 ELISA Kit, aldoa ELISA Kit, cb79 ELISA Kit, sb:cb79 ELISA Kit, wu:fa28b10 ELISA Kit, wu:fb10b11 ELISA Kit, ALDOA ELISA Kit, Aldo-1 ELISA Kit, Aldo1 ELISA Kit, RNALDOG5 ELISA Kit, hm:zeh0036 ELISA Kit, zgc:77696 ELISA Kit, aldolase, fructose-bisphosphate A ELISA Kit, aldolase a, fructose-bisphosphate, a ELISA Kit, aldolase, fructose-bisphosphate A S homeolog ELISA Kit, aldolase A, fructose-bisphosphate ELISA Kit, aldolase a, fructose-bisphosphate, b ELISA Kit, ALDOA ELISA Kit, aldoaa ELISA Kit, aldoa ELISA Kit, aldoa.S ELISA Kit, Aldoa ELISA Kit, aldoab ELISA Kit

Background: Alternative name: FBA, ALDO-A, Fructose 1,6 Bisphosphate Aldolase, Lung cancer antigen NY-LU-1, Muscle-type aldolase

Gene ID: 11674

UniProt: P05064

Pathways: Ribonucleoside Biosynthetic Process