LC3B ELISA Kit

Catalog No. ABIN5674808

Product Details LC3B ELISA Kit

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0068 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: Microtubule Associated Protein 1 Light Chain 3 Beta (MAP1LC3b) (MAP1LC3B Products)

Synonyms: ATG8F ELISA Kit, LC3B ELISA Kit, MAP1A/1BLC3 ELISA Kit, MAP1LC3B-a ELISA Kit, Map1lc3 ELISA Kit, Mpl3 ELISA Kit, zbs559 ELISA Kit, 1010001C15Rik ELISA Kit, Atg8 ELISA Kit, LC3b ELISA Kit, zgc:56434 ELISA Kit, wu:fb60g11 ELISA Kit, map1lc3b ELISA Kit, MGC76283 ELISA Kit, MAP1LC3B ELISA Kit, microtubule associated protein 1 light chain 3 beta ELISA Kit, microtubule-associated protein 1 light chain 3 beta ELISA Kit, microtubule associated protein 1 light chain 3 beta L homeolog ELISA Kit, MAP1LC3B ELISA Kit, Map1lc3b ELISA Kit, map1lc3b ELISA Kit, map1lc3b.L ELISA Kit

Background: Alternative name: MAP1A/1BLC3, ATG8F, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2

Gene ID: 81631

UniProt: Q9GZQ8

Pathways: Autophagy