UGCG ELISA Kit

Catalog No. ABIN5674820

Product Details UGCG ELISA Kit

Target: UGCG (UDP-Glucose Ceramide Glucosyltransferase (UGCG))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0115 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for UGCG

Target: UGCG (UDP-Glucose Ceramide Glucosyltransferase (UGCG))

Alternative Name: UDP Glucose Ceramide Glucosyltransferase (UGCG) (UGCG Products)

Synonyms: GCS ELISA Kit, GLCT1 ELISA Kit, XLCGT ELISA Kit, gcs ELISA Kit, glct1 ELISA Kit, ugcga ELISA Kit, xlcgt ELISA Kit, cb539 ELISA Kit, sb:cb539 ELISA Kit, zgc:112506 ELISA Kit, ugcg ELISA Kit, ugcgb ELISA Kit, AU043821 ELISA Kit, C80537 ELISA Kit, Epcs21 ELISA Kit, GlcT-1 ELISA Kit, Ugcgl ELISA Kit, UDP-glucose ceramide glucosyltransferase ELISA Kit, UDP-glucose ceramide glucosyltransferase L homeolog ELISA Kit, UDP-glucose ceramide glucosyltransferase S homeolog ELISA Kit, UGCG ELISA Kit, ugcg.L ELISA Kit, Ugcg ELISA Kit, ugcg ELISA Kit, ugcg.S ELISA Kit

Background: Alternative name: GCS, GLCT-1, Glucosylceramide Synthase, N-acylsphingosine D-glucosyltransferase

Gene ID: 7357

UniProt: Q16739