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DBP ELISA Kit

DBP Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
Catalog No. ABIN5674823
  • Target See all DBP ELISA Kits
    DBP (D Site of Albumin Promoter (Albumin D-Box) Binding Protein (DBP))
    Reactivity
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156 ng/mL - 10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Sample Type
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensitivity
    0051 ng/mL
    Components
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
    Top Product
    Discover our top product DBP ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target See all DBP ELISA Kits
    DBP (D Site of Albumin Promoter (Albumin D-Box) Binding Protein (DBP))
    Alternative Name
    D Site Of Albumin Promoter Binding Protein (DBP) (DBP Products)
    Synonyms
    DBP ELISA Kit, DABP ELISA Kit, Gc ELISA Kit, dbp ELISA Kit, tef ELISA Kit, D-box binding PAR bZIP transcription factor ELISA Kit, D site albumin promoter binding protein ELISA Kit, GC, vitamin D binding protein ELISA Kit, D site of albumin promoter (albumin D-box) binding protein L homeolog ELISA Kit, DBP ELISA Kit, Dbp ELISA Kit, GC ELISA Kit, dbp.L ELISA Kit
    Background
    Alternative name: DABP, TaxREB302, Albumin D box-binding protein, Albumin D-element-binding protein, Tax-responsive enhancer element-binding protein 302
    Gene ID
    13170
    UniProt
    Q60925
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