DAB1 ELISA Kit

Catalog No. ABIN5674825

Product Details DAB1 ELISA Kit

Target: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0139 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for DAB1

Target: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Alternative Name: Disabled Homolog 1 (DAB1) (DAB1 Products)

Synonyms: AI956902 ELISA Kit, C630028C02Rik ELISA Kit, scm ELISA Kit, scr ELISA Kit, scrambler ELISA Kit, yot ELISA Kit, disabled-1 ELISA Kit, LOC100232823 ELISA Kit, si:dkey-242m13.1 ELISA Kit, DAB1 ELISA Kit, zgc:136547 ELISA Kit, DAB1, reelin adaptor protein ELISA Kit, disabled 1 ELISA Kit, disabled homolog 1 ELISA Kit, Dab, reelin signal transducer, homolog 1a (Drosophila) ELISA Kit, Dab, reelin signal transducer, homolog 1b (Drosophila) ELISA Kit, DAB1 ELISA Kit, Dab1 ELISA Kit, LOC100232823 ELISA Kit, dab1a ELISA Kit, dab1b ELISA Kit

Gene ID: 1600

UniProt: O75553