EGFL7 ELISA Kit

Catalog No. ABIN5674827

Product Details EGFL7 ELISA Kit

Target: EGFL7 (EGF-Like-Domain, Multiple 7 (EGFL7))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 14.8 pg/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for EGFL7

Target: EGFL7 (EGF-Like-Domain, Multiple 7 (EGFL7))

Alternative Name: EGF Like Domain Protein, Multiple 7 (EGFL7) (EGFL7 Products)

Synonyms: egfl7 ELISA Kit, MGC53105 ELISA Kit, EGFL7 ELISA Kit, zgc:136669 ELISA Kit, zgc:152804 ELISA Kit, zneu1 ELISA Kit, xegfl7 ELISA Kit, MGC147423 ELISA Kit, VE-statin ELISA Kit, Zneu1 ELISA Kit, NEU1 ELISA Kit, RP11-251M1.2 ELISA Kit, VE-STATIN ELISA Kit, ZNEU1 ELISA Kit, EGF like domain multiple 7 S homeolog ELISA Kit, EGF like domain multiple 7 ELISA Kit, EGF-like-domain, multiple 7 ELISA Kit, EGF-like domain 7 ELISA Kit, egfl7.S ELISA Kit, EGFL7 ELISA Kit, egfl7 ELISA Kit, Egfl7 ELISA Kit

Background: Alternative name: ZNEU1, MEGF7, Zneu1, Vascular endothelial statin, EGF Like Domain Containing 7, Epidermal growth factor-like protein 7, Multiple epidermal growth factor-like domains protein 7

Gene ID: 51162

UniProt: Q9UHF1