CPB1 ELISA Kit

Catalog No. ABIN5674875

Product Details CPB1 ELISA Kit

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Sensitivity: 0055 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for CPB1

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Alternative Name: Carboxypeptidase B1, Tissue (CPB1) (CPB1 Products)

Synonyms: wu:fb60d07 ELISA Kit, wu:fb69b08 ELISA Kit, CPB1 ELISA Kit, pCPB ELISA Kit, CPB ELISA Kit, PASP ELISA Kit, PCPB ELISA Kit, Cpb ELISA Kit, 0910001A18Rik ELISA Kit, 1810063F02Rik ELISA Kit, 2210008M23Rik ELISA Kit, AI504870 ELISA Kit, carboxypeptidase B1 (tissue) ELISA Kit, carboxypeptidase B1 ELISA Kit, carboxypeptidase B1 S homeolog ELISA Kit, carboxypeptidase B ELISA Kit, cpb1 ELISA Kit, CPB1 ELISA Kit, cpb1.S ELISA Kit, CpipJ_CPIJ010799 ELISA Kit, CpipJ_CPIJ010801 ELISA Kit, VDBG_02663 ELISA Kit, LOC100562609 ELISA Kit, Cpb1 ELISA Kit

Background: Alternative name: CPB, PCPB, PASP, Pancreas-specific protein

Gene ID: 1360

UniProt: P15086