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EPHX1 ELISA Kit

EPHX1 Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5674887
  • Target See all EPHX1 ELISA Kits
    EPHX1 (Epoxide Hydrolase 1, Microsomal (Xenobiotic) (EPHX1))
    Reactivity
    • 2
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    62.5 pg/mL - 4000 pg/mL
    Minimum Detection Limit
    62.5 pg/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensitivity
    28.6 pg/mL
    Components
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
    Top Product
    Discover our top product EPHX1 ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target See all EPHX1 ELISA Kits
    EPHX1 (Epoxide Hydrolase 1, Microsomal (Xenobiotic) (EPHX1))
    Alternative Name
    Epoxide Hydrolase 1, Microsomal (EPHX1) (EPHX1 Products)
    Synonyms
    NV13267 ELISA Kit, DRB1 ELISA Kit, DSRNA-BINDING PROTEIN 1 ELISA Kit, F21M12.9 ELISA Kit, F21M12_9 ELISA Kit, HYPONASTIC LEAVES 1 ELISA Kit, AI195553 ELISA Kit, Eph-1 ELISA Kit, Eph1 ELISA Kit, mEH ELISA Kit, EPHX ELISA Kit, EPOX ELISA Kit, HYL1 ELISA Kit, MEH ELISA Kit, zgc:56126 ELISA Kit, ephx ELISA Kit, epox ELISA Kit, meh ELISA Kit, MEH8 ELISA Kit, epoxide hydrolase 1 ELISA Kit, epoxide hydrolase 1, microsomal (xenobiotic) ELISA Kit, Epoxide hydrolase 1 ELISA Kit, dsRNA-binding domain-like superfamily protein ELISA Kit, epoxide hydrolase 1, microsomal ELISA Kit, epoxide hydrolase 1, microsomal (xenobiotic) L homeolog ELISA Kit, EPHX1 ELISA Kit, Eh1 ELISA Kit, hyep ELISA Kit, HYL1 ELISA Kit, Ephx1 ELISA Kit, ephx1 ELISA Kit, ephx1.L ELISA Kit
    Background
    Alternative name: Epoxide hydratase, Microsomal epoxide hydrolase, Eph-1, MEH8
    Gene ID
    2052
    UniProt
    P07099
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