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RNLS ELISA Kit

RNLS Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Catalog No. ABIN5674910
  • Target See all RNLS ELISA Kits
    RNLS (Renalase, FAD-Dependent Amine Oxidase (RNLS))
    Reactivity
    • 2
    • 2
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    1.56 ng/mL - 100 ng/mL
    Minimum Detection Limit
    1.56 ng/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Sensitivity
    0.69 ng/mL
    Components
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target See all RNLS ELISA Kits
    RNLS (Renalase, FAD-Dependent Amine Oxidase (RNLS))
    Alternative Name
    Renalase (RNLS) (RNLS Products)
    Synonyms
    zgc:92286 ELISA Kit, C10orf59 ELISA Kit, RENALASE ELISA Kit, 6530404N21Rik ELISA Kit, AI452315 ELISA Kit, AW060440 ELISA Kit, RGD1309804 ELISA Kit, renalase, FAD dependent amine oxidase ELISA Kit, renalase, FAD-dependent amine oxidase ELISA Kit, RNLS ELISA Kit, rnls ELISA Kit, Rnls ELISA Kit
    Background
    Alternative name: MAO-C, C10orf60, FAD-Dependent Amine Oxidase, Monoamine oxidase-C, alpha-NAD(P)H oxidase/anomerase
    Gene ID
    67795
    UniProt
    A7RDN6
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