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PDE1C ELISA Kit

PDE1C Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
Catalog No. ABIN5674919
  • Target See all PDE1C ELISA Kits
    PDE1C (phosphodiesterase 1C, Calmodulin-Dependent 70kDa (PDE1C))
    Reactivity
    • 2
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156 ng/mL - 10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Sample Type
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensitivity
    0042 ng/mL
    Components
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target See all PDE1C ELISA Kits
    PDE1C (phosphodiesterase 1C, Calmodulin-Dependent 70kDa (PDE1C))
    Alternative Name
    Phosphodiesterase 1C, Calmodulin Dependent (PDE1C) (PDE1C Products)
    Synonyms
    CG14940 ELISA Kit, CG14942 ELISA Kit, CG14943 ELISA Kit, CG14944 ELISA Kit, CG31757 ELISA Kit, CG31758 ELISA Kit, CG42325 ELISA Kit, CG44007 ELISA Kit, Dmel\\CG44007 ELISA Kit, Dmel_CG14940 ELISA Kit, Dmel_CG14943 ELISA Kit, Dmel_CG14944 ELISA Kit, Dmel_CG31757 ELISA Kit, Dmel_CG31758 ELISA Kit, Dmel_CG42325 ELISA Kit, PDE1C ELISA Kit, PDE1c ELISA Kit, GB13690 ELISA Kit, Hcam3 ELISA Kit, Phosphodiesterase 1c ELISA Kit, phosphodiesterase 1C ELISA Kit, calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A ELISA Kit, calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C ELISA Kit, phosphodiesterase 1C, calmodulin-dependent b ELISA Kit, Pde1c ELISA Kit, PDE1C ELISA Kit, LOC724389 ELISA Kit, LOC100019026 ELISA Kit, pde1cb ELISA Kit
    Background
    Alternative name: Hcam3, Cam-PDE 1C, Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C
    Gene ID
    81742
    UniProt
    Q63421
    Pathways
    EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma
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