Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

SPTLC1 ELISA Kit

SPTLC1 Reactivity: Human Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Tissue Homogenate
Catalog No. ABIN5674929
  • Target See all SPTLC1 ELISA Kits
    SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))
    Reactivity
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    78.12 pg/mL - 5000 pg/mL
    Minimum Detection Limit
    78.12 pg/mL
    Application
    ELISA
    Sample Type
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensitivity
    27 ng/mL
    Components
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
    Featured
    Discover our best selling SPTLC1 ELISA Kit
    Top Product
    Discover our top product SPTLC1 ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target See all SPTLC1 ELISA Kits
    SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))
    Abstract
    SPTLC1 Products
    Synonyms
    ATSPT1 ELISA Kit, SERINE PALMITOYLTRANSFERASE 1 ELISA Kit, serine palmitoyltransferase 1 ELISA Kit, Afu6g00300 ELISA Kit, HSAN1 ELISA Kit, HSN1 ELISA Kit, LBC1 ELISA Kit, LCB1 ELISA Kit, SPT1 ELISA Kit, SPTI ELISA Kit, wu:fc75h04 ELISA Kit, wu:fp41c08 ELISA Kit, zgc:112247 ELISA Kit, AW552086 ELISA Kit, C77762 ELISA Kit, E030036H05 ELISA Kit, Lcb1 ELISA Kit, RGD1306617 ELISA Kit, Spt1 ELISA Kit, serine palmitoyltransferase 1 ELISA Kit, Serine palmitoyltransferase 1 ELISA Kit, serine palmitoyltransferase long chain base subunit 1 ELISA Kit, serine palmitoyltransferase, long chain base subunit 1 ELISA Kit, serine palmitoyltransferase, long chain base subunit 1 L homeolog ELISA Kit, SPT1 ELISA Kit, AFUA_6G00300 ELISA Kit, PAS_FragB_0040 ELISA Kit, SPTLC1 ELISA Kit, sptlc1 ELISA Kit, sptlc1.L ELISA Kit, Sptlc1 ELISA Kit
    Background
    Alternative name: HSAN, HSAN1, HSN1, LBC1, LCB1, SPT1, SPTI, Hereditary Sensory Neuropathy,Type 1, Long chain base biosynthesis protein 1, Serine-palmitoyl-CoA transferase 1
    Gene ID
    10558
    UniProt
    O15269
You are here:
Support