MASP2 ELISA Kit

Catalog No. ABIN5674934

Product Details MASP2 ELISA Kit

Target: MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Sensitivity: 0067 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for MASP2

Target: MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))

Alternative Name: Mannose Associated Serine Protease 2 (MASP2) (MASP2 Products)

Synonyms: MAP19 ELISA Kit, MASP-2 ELISA Kit, MASP1P1 ELISA Kit, sMAP ELISA Kit, MAp19 ELISA Kit, mannan binding lectin serine peptidase 2 ELISA Kit, mannan-binding lectin serine peptidase 2 ELISA Kit, MASP2 ELISA Kit, Masp2 ELISA Kit

Background: Alternative name: Mannose-binding protein-associated serine protease 2, mannan-Binding Lectin Serine Peptidase 2, MBL-associated serine protease 2

Gene ID: 17175

UniProt: Q91WP0

Pathways: Complement System