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GATA1 ELISA Kit

GATA1 Reactivity: Human Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
Catalog No. ABIN5690754
  • Target See all GATA1 ELISA Kits
    GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))
    Reactivity
    • 7
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    DNA-Binding ELISA
    Application
    ELISA
    Purpose
     Human GATA-1 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical GATA1 binding sequences to semi-quantitatively detect active GATA1 in lysates or nuclear extracts.
    Sample Type
    Cell Lysate, Nuclear Extract
    Analytical Method
    Semi-Quantitative
    Specificity
    The olionucleotide/antibody pair provided in this kit recognizes human GATA-1 in whole lysates and nuclear extracts.
    Characteristics
    • Specific transcription factor-DNA binding assay
    • Perfect alternative to EMSA
    • Easy to perform in an ELISA format
    • Non-radioactive assay
    • High throughput (96 well plate format)
    • Assay can be completed within 5 hours
    Components
    • 96-well Strip Microplate pre-coated with DNA probes
    • DNA Binding Buffer
    • Positive Control Sample
    • Specific Competitor DNA probe
    • Non-specific Competitor DNA probe
    • Assay Reagent
    • DTT
    • Wash Buffer
    • Primary Antibody
    • HRP-conjugated Secondary Antibody
    • TMB One-Step Substrate Reagent
    • Stop Solution
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Absorbent paper
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared HRP-secondary antibody to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Storage Comment
    Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
    Expiry Date
    6 months
  • Target See all GATA1 ELISA Kits
    GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))
    Alternative Name
    GATA1 (GATA1 Products)
    Synonyms
    ERYF1 ELISA Kit, GATA-1 ELISA Kit, GF-1 ELISA Kit, GF1 ELISA Kit, NF-E1 ELISA Kit, NFE1 ELISA Kit, XLANP ELISA Kit, XLTDA ELISA Kit, XLTT ELISA Kit, Gata-1 ELISA Kit, Gf-1 ELISA Kit, eryf1 ELISA Kit, Eryf1 ELISA Kit, GATA1 ELISA Kit, gata-1 ELISA Kit, Gata1 ELISA Kit, si:dkeyp-77f7.5 ELISA Kit, gata1 ELISA Kit, gata1a-A ELISA Kit, xGATA-1 ELISA Kit, xgata1 ELISA Kit, XGATA-1B ELISA Kit, gata1b-A ELISA Kit, gta1 ELISA Kit, zg1 ELISA Kit, NF-E1a ELISA Kit, GATA binding protein 1 ELISA Kit, GATA binding protein 1 (globin transcription factor 1) ELISA Kit, amidase ELISA Kit, aspartyl/glutamyl-tRNA amidotransferase subunit A ELISA Kit, putative amidase ELISA Kit, GatA1 ELISA Kit, glutamyl-tRNA amidotransferase subunit A ELISA Kit, hematopietic transcription factor GATA-1 ELISA Kit, GATA binding protein 1b ELISA Kit, GATA binding protein 1 L homeolog ELISA Kit, GATA binding protein 1 S homeolog ELISA Kit, GATA binding protein 1a ELISA Kit, erythroid transcription factor ELISA Kit, GATA1 ELISA Kit, Gata1 ELISA Kit, gatA1 ELISA Kit, gata-1 ELISA Kit, gata1b ELISA Kit, gata1.L ELISA Kit, gata1.S ELISA Kit, gata1a ELISA Kit, LOC108348091 ELISA Kit
    Gene ID
    2623
    UniProt
    P15976
    Pathways
    Cellular Response to Molecule of Bacterial Origin
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