+1 877 302 8632
+1 888 205 9894 (Toll-free)


SP1 Reactivity: Human Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
Catalog No. ABIN5690763
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 2 to 3 Business Days
  • Target See all SP1 ELISA Kits
    SP1 (Sp1 Transcription Factor (SP1))
    • 7
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Detection Method
    Method Type
    DNA-Binding ELISA
     Human Sp1 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical Sp1 binding sequences to semi-quantitatively detect active Sp1 in lysates or nuclear extracts.
    Sample Type
    Cell Lysate, Nuclear Extract
    Analytical Method
    The olionucleotide/antibody pair provided in this kit recognizes human Sp1 in whole lysates and nuclear extracts.
    • Specific transcription factor-DNA binding assay
    • Perfect alternative to EMSA
    • Easy to perform in an ELISA format
    • Non-radioactive assay
    • High throughput (96 well plate format)
    • Assay can be completed within 5 hours
    • 96-well Strip Microplate pre-coated with DNA probes
    • DNA Binding Buffer
    • Positive Control Sample
    • Specific Competitor DNA probe
    • Non-specific Competitor DNA probe
    • Assay Reagent
    • DTT
    • Wash Buffer
    • Primary Antibody
    • HRP-conjugated Secondary Antibody
    • TMB One-Step Substrate Reagent
    • Stop Solution
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Absorbent paper
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Discover our best selling SP1 ELISA Kit
  • Plate
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared HRP-secondary antibody to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    For Research Use only
  • Storage
    4 °C
    Storage Comment
    Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
    Expiry Date
    6 months
  • Target See all SP1 ELISA Kits
    SP1 (Sp1 Transcription Factor (SP1))
    Alternative Name
    Sp1 (SP1 Products)
    fc18h09 ELISA Kit, zgc:85950 ELISA Kit, wu:fc18h09 ELISA Kit, SP1 ELISA Kit, 1110003E12Rik ELISA Kit, AA450830 ELISA Kit, AI845540 ELISA Kit, Sp1-1 ELISA Kit, sp1 transcription factor ELISA Kit, Sp1 transcription factor ELISA Kit, Sp1 transcription factor L homeolog ELISA Kit, trans-acting transcription factor 1 ELISA Kit, sp1 ELISA Kit, SP1 ELISA Kit, Sp1 ELISA Kit, sp1.L ELISA Kit
    Gene ID
    Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction
You are here: