ACP6 ELISA Kit

Catalog No. ABIN5690814

Product Details ACP6 ELISA Kit

Target: ACP6 (Acid Phosphatase 6, Lysophosphatidic (ACP6))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ACP6 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human ACP6.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for ACP6

Target: ACP6 (Acid Phosphatase 6, Lysophosphatidic (ACP6))

Alternative Name: ACP6 (ACP6 Products)

Synonyms: ACP6 ELISA Kit, im:7147584 ELISA Kit, zgc:172268 ELISA Kit, MGC146066 ELISA Kit, ACPL1 ELISA Kit, LPAP ELISA Kit, PACPL1 ELISA Kit, 5730559A09Rik ELISA Kit, AU022842 ELISA Kit, mPACPL1 ELISA Kit, acid phosphatase 6, lysophosphatidic ELISA Kit, acid phosphatase 6, lysophosphatidic S homeolog ELISA Kit, ACP6 ELISA Kit, acp6 ELISA Kit, acp6.S ELISA Kit, Acp6 ELISA Kit

Gene ID: 51205

UniProt: Q9NPH0