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BNIP3 ELISA Kit

BNIP3 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN5690831
$764.50
Plus shipping costs $45.00
96 tests
Shipping to: United States
Delivery in 31 to 41 Business Days
  • Target See all BNIP3 ELISA Kits
    BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))
    Reactivity
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human BNIP3 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human BNIP3.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    6 months
  • Target See all BNIP3 ELISA Kits
    BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))
    Alternative Name
    BNIP3 (BNIP3 Products)
    Synonyms
    NIP3 ELISA Kit, Nip3 ELISA Kit, BCL2 interacting protein 3 ELISA Kit, BCL2/adenovirus E1B interacting protein 3 ELISA Kit, BNIP3 ELISA Kit, Bnip3 ELISA Kit
    Gene ID
    664
    UniProt
    Q12983
    Pathways
    Autophagy, Brown Fat Cell Differentiation
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